Lack of artemisinin resistance in Plasmodium falciparum in northwest Benin after 10 years of use of artemisinin-based combination therapy

Lack of artemisinin resistance in Plasmodium falciparum in northwest Benin after 10 years of use of artemisinin-based combination therapy

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Aim: In Benin, artemisinin-based combination therapy (ACT) has been recommended as the first-line treatment for uncomplicated Plasmodium falciparum malaria since 2004.The emergence in Southeast Asia of parasites that are resistant to artemisinins poses a serious threat to global control of this disease.The presence of artemisinin resistance genotypes in parasite populations in Benin is currently unknown.The present study investigated the prevalence of relevant K13-propeller gene polymorphisms in parasite isolates from the north-western region of Benin.

Method: Plasmodium falciparum isolates were collected from children with a confirmed diagnosis of malaria aged 6 months to 5 years in two towns, Cobly and AEG SteamBake BPS552020M Electric Oven - Stainless Steel 60cm Djougou, in the north-western part PYO Supplies of Benin.The study was conducted during the rainy season from July to November 2014 in local health facilities.The K13-propeller gene was amplified in parasite isolates using nested PCR and subsequently sequenced.Results: A total of 108 children were recruited into the study.

The efficiency of amplification reactions was 72% (78/108).The propeller domain of the K13 gene was successfully sequenced in 78 P.falciparum isolates; all of them were wild type with no polymorphisms detectable.Conclusion: The absence of mutations in the K13 gene indicates that P.

falciparum parasite populations in the study area are still fully susceptible to artemisinins.